Hmgb1-/- and +/+ embryonic fibroblast immortalized with large T antigen [HM-201]
HMGBiotech distributes the knockout cells generated by the laboratory of Prof. Marco E. Bianchi for research purposes. San Raffaele Research Institute remains the owner of the intellectual property associated to these cells, and requires anyone interested in acquiring them to sign an MTA. Please send a mail to firstname.lastname@example.org for MTA processing.
The cells are shipped in dry ice. Since they are living biological material, they will be checked at customs. Therefore, communication between HMGBiotech and the buyer is required to optimize the delivery.
These are non-syngenic, and derive from a F2 cross of 129Sv and C57Bl/6 mice (Calogero et al. The lack of chromosomal protein HMG1 does not disrupt cell growth, but causes lethal hypoglycaemia in newborn mice. Nature Genet 1999, 22: 276-80).
These lines are called VA1 (Hmgb1+/+) and C1 (Hmgb1-/-). Please be advised that these cells contain HMGB2, which in most instances is redundant to HMGB1. Hmgb1-/- cells grow more slowly than Hmgb1+/+ cells, and are less resistant to freezing.
Publications where this product was used:
Scaffidi et al. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature 2002, 418: 191-5
other publications: 1, 2, 3