Hmgb1-/- and +/+ embryonic fibroblasts immortalized with the 3T3 method [HM-221]
HMGBiotech distributes the knockout cells generated by the laboratory of Prof. Marco E. Bianchi for research purposes. San Raffaele Research Institute remains the owner of the intellectual property associated to these cells, and requires anyone interested in acquiring them to sign an MTA.
Please send a mail to firstname.lastname@example.org for MTA processing.
The cells are shipped in dry ice. Since they are living biological material, they will be checked at customs. Therefore, communication between HMGBiotech and the buyer is required to optimize the delivery.
These Hmgb1+/+ and Hmgb1-/- MEFs were obtained from sib embryos from crosses of Hmgb1 heterozygotes on a pure BALB/c genetic background (>10 backcrosses), and grown according to the 3T3 protocol, which prescribes splitting 1:3 the growing cells every 3 days for 3 months.
We suggest to split the cells, by trypsinization, when they reach 80% confluence.