pEGFP-HMGB1 plasmid (20 µg) [HM-302]
Plasmid pEGFP-HMGB1 was obtained by inserting the coding sequence for the rat HMGB1 into pEGFP-N1 (Clontech) using the EcoRi and SacII restriction sites (Scaffidi et al. Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature 2002, 418: 191-5). It expresses HMGB1-GFP, which is rat HMGB1 (at the N terminus) fused to GFP (at the C terminus). This protein is fully functional and is functionally equivalent to unmodified HMGB1 (Pallier et al. Association of chromatin proteins HMGB1 and HMGB2 with mitotic chromosomes. Mol Biol Cell 2003, 14: 3414-26).
Publications where this plasmid was used:
Bonaldi et al. Monocytic cells hyperacetylate chromatin protein HMGB1 to redirect it towards secretion. EMBO J 2003, 22: 5551-60
Agresti et al. GR and HMGB1 interact only within chromatin and influence each other’s residence time. Mol Cell 2005, 18: 109-121