BoxA from HMGB1, human & mouse, LPS-Free
BoxA is one of the highly conserved DNA binding domains of HMGB1 protein (HMG boxes), and inhibits HMGB1 effects in vivo.
The BoxA we provide has no difference from the sequence of the human or mouse HMGB1, and as a self-antigen elicits very low immunological responses. If you need large amounts of BoxA for preclinical studies, please enquire for special prices at info@hmgbiotech.com.
Large amounts of BoxA can also be provided on a collaborative basis.
BoxA protects against sepsis in a mouse peritonitis model (Yang et al. Reversing established sepsis with antagonists of endogenous high-mobility group box 1. Proc Natl Acad Sci USA 2004, 101: 296-301), and from hepatitis in a mouse model of HBV infection (Sitia et al. Treatment with HMGB1 inhibitors diminishes CTL-induced liver disease in HBV transgenic mice. J Leukoc Biol 2007, 81:100-7).
Dendritic cells promote their own maturation and T cell proliferation, survival and Th1 differentiation by secreting HMGB1, and BoxA inhibits these activities (Dumitriu et al. Release of HMGB1 by dendritic cells controls T cell activation via the receptor for advanced glycation end products (RAGE). J Immunol 2005, 174:7506-15).
BoxA also inhibits the chemotactic activity of HMGB1 and the mitogenic activity of HMGB1 on stem cells.
BoxA from HMGB1 is produced in E.coli from an expression plasmid coding for the unmodified mammalian sequence, which is totally identical in human and mouse (Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40: 10254-61).
It has the sequence:
[“MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKF” ]
Molecular Mass: BoxA from HMGB1 consists of 89 amino acid and has a calculated molecular mass of approximately 10.4 kDa.
Purity: The purified protein is >95% homogeneous (electrophoresis). It contains no nucleic acids.
Endotoxin Level: The purified protein is free from LPS (Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL). The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure. The remaining traces of Triton X-114 can be removed upon request.
Buffer & Reconstitution: the lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: the protein is shipped lyophilized. Once resuspended can be stored frozen at -20°C..
This product is intended for research only, and cannot be used on humans.
Publications:
- An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling
- Lipopolysaccharide-Activated Canine Platelets Upregulate High Mobility Group Box-1 via Toll-Like Receptor 4
- A RAGE-antagonist peptide potentiates polymeric micelle-mediated intracellular delivery of plasmid DNA for acute lung injury gene therapy
- The Time-Course of Antioxidant Irisin Activity: Role of the Nrf2/HO-1/HMGB1 Axis
- Lipopolysaccharide-regulated secretion of soluble and vesicle-based proteins from a panel of colorectal cancer cell lines
- High-mobility group box protein-1 induces acute pancreatitis through activation of neutrophil extracellular trap and subsequent production of IL-1β
- Increased cell-free fetal DNA release after apoptosis and sterile inflammation in human trophoblast cells
- Photodynamic Therapy in Combination with the Hepatitis B Core Virus-like Particles (HBc VLPs) to Prime Anticancer Immunity for Colorectal Cancer Treatment
- The protective mechanism of salidroside modulating miR-199a-5p/TNFAIP8L2 on lipopolysaccharide-induced MLE-12 cells
- High Mobility Group Proteins in Sepsis
- The Role of High Mobility Group Box 1 in Ischemic Stroke
- High mobility group A proteins as tumor markers
- Reappraisal of oxidized HMGB1 as a mediator and biomarker
- Adenovirus protein VII binds the A-box of HMGB1 to repress interferon responses
- HMGB1 cleavage by complement C1s and its potent anti-inflammatory product
- Research on the interaction of astragaloside IV and calycosin in Astragalus membranaceus with HMGB1
- HMGB1 induces hepcidin upregulation in astrocytes and causes an acute iron surge and subsequent ferroptosis in the postischemic brain
- Role of Histiocyte-Derived frHMGB1 as a Facilitator in Non-Canonical Pyroptosis of Monocytes/Macrophages in Lethal Sepsis
- HMGB1 cleavage by complement C1s and its potent anti-inflammatory product
- Discovery of 5,5′-Methylenedi-2,3-Cresotic Acid as a Potent Inhibitor of the Chemotactic Activity of the HMGB1·CXCL12 Heterocomplex Using Virtual Screening and NMR Validation
- Inhibition of inflammatory liver injury by the HMGB1-A box through HMGB1/TLR-4/NF-κB signaling in an acute liver failure mouse model
- High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy
- Use of an antagonist of HMGB1 in mice affected by malignant mesothelioma: a preliminary ultrasound and optical imaging study
- High mobility group box 1 inhibition by BoxA attenuates ovalbumin-induced allergic rhinitis in mice
- Disulfide HMGB1 acts via TLR2/4 receptors to reduce the numbers of oligodendrocyte progenitor cells after traumatic injury in vitro
- High mobility group box-1 (HMGB1) antagonist BoxA suppresses status epilepticus-induced neuroinflammatory responses associated with Toll-like receptor 2/4 down-regulation in rats
- Inhibition of Cerebral High-Mobility Group Box 1 Protein Attenuates Multiple Organ Damage and Improves T Cell-Mediated Immunity in Septic Rats
- Early antagonism of cerebral high mobility group box-1 protein is benefit for sepsis induced brain injury
- The Role of Box A of HMGB1 in Enhancing Stem Cell Properties of Human Mesenchymal Cells: A Novel Approach for the Pursuit of Anti-aging Therapy
- CARDIOPULMONARY BYPASS-DERIVED PLASMA EXOSOMAL HMGB1 CONTRIBUTES TO ALVEOLAR EPITHELIAL CELL NECROPTOSIS VIA mtDNA/CGAS/STING PATHWAY
- Synthesized HMGB1 peptide prevents the progression of inflammation, steatosis, fibrosis, and tumor occurrence in a non-alcoholic steatohepatitis mouse model
- The roles of HMGB1-produced DNA gaps in DNA protection and aging biomarker reversal
- Targeting Inflammation Driven by HMGB1 in Bacterial Keratitis-A Review
- Synthesized HMGB1 peptide attenuates liver inflammation and suppresses fibrosis in mice
- IL-1β and HMGB1 are anti-neurogenic to endogenous neural stem cells in the sclerotic epileptic human hippocampus
- Sitia et al. Treatment with HMGB1 inhibitors diminishes CTL-induced liver disease in HBV transgenic mice. J Leukoc Biol 2007, 81:100-7.
- Andrassy et al. High-mobility group box-1 in ischemia-reperfusion injury of the heart. Circulation 2008, 117:3216-26.
- Muhammad et al. The HMGB1 receptor RAGE mediates ischemic brain damage. J Neurosci 2008, 28:12023-31.
- Urbonaviciute et al. Induction of inflammatory and immune responses by HMGB1-nucleosome complexes: implications SLE. J Exp Med 2008, 205:3007-18.
- Maroso et al. Toll-like receptor 4 and high mobility group box-1 are involved in ictogenesis and can be targeted to reduce seizures. Nature Medicine 2010, 16:413-9.
Complete Name: BoxA from High Mobility Group 1
Other Names: A-Box HMGB1, Box-A from HMGB1, BoxA from High Mobility Group 1
