BoxB from HMGB1, human & mouse

BoxB from HMGB1, human & mouse, LPS-Free

BoxB is one of the DNA binding domains of HMGB1 protein (HMG boxes).

BoxB from HMGB1 is produced in E.coli (Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40: 10254-61) from an expression plasmid coding for the mammalian sequence, which is totally identical in human and mouse, preceded by four amino acids (MARI) that have been added for the purpose of expression in bacteria (Bianchi et al. The DNA binding site of HMG1 protein is composed of two similar segments (HMG boxes), both of which have counterparts in other eukaryotic regulatory proteins. EMBO J 1992, 11: 1055-63).
It has the sequence:

[“MARIDPNAPK RPPSAFFLFC SEYRPKIKGE HPGLSIGDVA KKLGEMWNNT AADDKQPYEK KAAKLKEKYE KDIAAYRAKG KPDAAKKGVV” ]

Molecular Mass: Box B from HMGB1 consists of 90 aminoacid residues and has a calculated molecular mass of approximately 10.0 kDa.
Purity: The purified protein is >95% homogeneous (electrophoresis). It contains no nucleic acids.
Endotoxin Level: The purified protein is free from LPS (Pierce™ Chromogenic Endotoxin Quant Kit, <0.1 EU/mL). The product contains <0.006% v/v of Triton X-114 due to LPS removal procedure. The remaining traces of Triton X-114 can be removed upon request.
Buffer & Reconstitution: the lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5mM DTT.
Storage: the protein is shipped lyophilized. Once resuspended can be stored frozen at -20°C.

This product is intended for research only, and cannot be used on humans.

Publications:

• An 8-Hydroxy-Quinoline Derivative Protects Against Lipopolysaccharide-Induced Lethality in Endotoxemia by Inhibiting HMGB1-Mediated Caspase-11 Signaling
• Lipopolysaccharide-Activated Canine Platelets Upregulate High Mobility Group Box-1 via Toll-Like Receptor 4
• High-mobility group box protein-1 induces acute pancreatitis through activation of neutrophil extracellular trap and subsequent production of IL-1β
• Increased cell-free fetal DNA release after apoptosis and sterile inflammation in human trophoblast cells
• Photodynamic Therapy in Combination with the Hepatitis B Core Virus-like Particles (HBc VLPs) to Prime Anticancer Immunity for Colorectal Cancer Treatment
• The protective mechanism of salidroside modulating miR-199a-5p/TNFAIP8L2 on lipopolysaccharide-induced MLE-12 cells
• A RAGE-antagonist peptide potentiates polymeric micelle-mediated intracellular delivery of plasmid DNA for acute lung injury gene therapy
• The Time-Course of Antioxidant Irisin Activity: Role of the Nrf2/HO-1/HMGB1 Axis
• Lipopolysaccharide-regulated secretion of soluble and vesicle-based proteins from a panel of colorectal cancer cell lines
• High-mobility group box1 as an amplifier of immune response and target for treatment in Aspergillus fumigatus keratitis
• The HMGB1-2 Ovarian Cancer Interactome. The Role of HMGB Proteins and Their Interacting Partners MIEN1 and NOP53 in Ovary Cancer and Drug-Response
• Elevated HMGB1 promotes the malignant progression and contributes to cisplatin resistance of non-small cell lung cancer
• The Role of High Mobility Group Box B-1 in the Prognosis of Colorectal Cancer Based on the Changes in the Intestinal Mucosal Barrier
• Interactions between N- and C-terminal domains of the Saccharomyces cerevisiae high-mobility group protein HMO1 are required for DNA bending
• Boxb mediate BALB/c mice corneal inflammation through a TLR4/MyD88-dependent signaling pathway in Aspergillus fumigatus keratitis
• Inhibition of LOX-1 alleviates the proinflammatory effects of high-mobility group box 1 in Aspergillus fumigatus keratitis
• N-glycosylation of High Mobility Group Box 1 protein (HMGB1) modulates the interaction with glycyrrhizin: A molecular modeling study
• High mobility group A proteins as tumor markers
• The Role of High Mobility Group Box 1 in Ischemic Stroke
• High Mobility Group Proteins in Sepsis
• PCV2 Induces Reactive Oxygen Species To Promote Nucleocytoplasmic Translocation of the Viral DNA Binding Protein HMGB1 To Enhance Its Replication
• Interaction of fumigaclavine C with High Mobility Group Box 1 protein (HMGB1) and its DNA complex: A computational approach
• Expression profiling and inflammatory activation analysis of high-mobility group box 1 in Schizothorax prenanti
• Exogenous Protein Delivery of Ionic Liquid-Mediated HMGB1 Coating on Titanium Implants
• Reappraisal of oxidized HMGB1 as a mediator and biomarker
• Research on the interaction of astragaloside IV and calycosin in Astragalus membranaceus with HMGB1
• Hypermethylation of RNF125 promotes autophagy-induced oxidative stress in asthma by increasing HMGB1 stability
• HMGB1 cleavage by complement C1s and its potent anti-inflammatory product
• Discovery of 5,5′-Methylenedi-2,3-Cresotic Acid as a Potent Inhibitor of the Chemotactic Activity of the HMGB1·CXCL12 Heterocomplex Using Virtual Screening and NMR Validation
• Inhibition of inflammatory liver injury by the HMGB1-A box through HMGB1/TLR-4/NF-κB signaling in an acute liver failure mouse model

Download BoxB Datasheet

Complete Name: BoxB from High Mobility Group 1
Other Names: B-Box HMGB1, Box-B from HMGB1, BoxB from High Mobility Group 1