HMGB1 is produced in E.coli from an expression plasmid coding for the human protein (Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40: 10254-61):
It has the sequence:
“MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE” ]
Molecular Mass: HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24,8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains.
Purity: The purified protein is >95% homogeneous (electrophoresis and mass spectrometry).
Activity: This preparation has low cytokine activity, but is fully active as a DNA binding protein or as an antigen.
Buffer: 50 mM HEPES buffer pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: We advise to keep the product frozen at -20°C.
This product is intended for research only, and cannot be used on humans.
Publications:
- The Role of High Mobility Group Box 1 in Ischemic Stroke
- High mobility group A proteins as tumor markers
- High Mobility Group Proteins in Sepsis
- The Roles of High Mobility Group Box 1 in Cerebral Ischemic Injury
- MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1
- Transposon dynamics in the emerging oilseed crop Thlaspi arvense
- Comparative analyses of the Smith-Magenis syndrome protein RAI1 in mice and common marmoset monkeys
- Intestinal epithelia and myeloid immune cells shape colitis severity and colorectal carcinogenesis via High-mobility group box protein 1
- Toxoplasma gondii Infection Induces High Mobility Group Box 1 Released from Mouse Macrophages
- Impact of folic acid supplementation on ischemia‒reperfusion-induced kidney injury in rats: folic acid prophylactic role revisited
- Co-existence of anti-glutamic acid decarboxylase-65 and anti-sry-like high-mobility group box receptor antibody-associated autoimmune encephalitis: A rare case report
- High Mobility Group Box 1 Gene Polymorphism and Serum High Mobility Group Box 1, Interleukin 1 Beta, and Alpha-Klotho Crosstalk in Severe COVID-19 Patients
- HO-1 attenuates testicular ischaemia/reperfusion injury by activating the phosphorylated C-jun-miR-221/222-TOX pathway
- MicroRNA-146a gene transfer ameliorates senescence and senescence-associated secretory phenotypes in tendinopathic tenocytes
- Therapeutic effects of anti-diabetic drugs on traumatic brain injury
- ASSOCIATION BETWEEN HIGH MOBILITY GROUP BOX 1 PROTEIN GENE (rs41369348) POLYMORPHISM AND IMMUNOGLOBULIN A VASCULITIS IN CHILDREN
- Mollica L et al. Glycyrrhizin binds to High Mobility Group Box 1 protein (HMGB1) and inhibits its cytokine activities. Chem Biol 2007, 14: 431-41
Download HMGB1 for Biochemestry Datasheet
Complete Name: High Mobility Group 1
Other Names: Amphoterin-1, High mobility group protein 1,HMG-1, SBP-1, high mobility group protein B1, high-mobility group box 1, Amphoterin, HMG1, HMGB1, SBP-1, recombinant HMGB1