HMGB1 is produced in E.coli from an expression plasmid coding for the human protein (Müller et al. Thermodynamics of HMGB1 interaction with duplex DNA. Biochemistry 2001, 40: 10254-61):
It has the sequence:
“MGKGDPKKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE” ]
Molecular Mass: HMGB1 consists of 215 amino acid residues and has a calculated molecular mass of approximately 24,8 kDa. It migrates at a position of approximately 30 kD in SDS-PAGE gels, possibly because of the unusual number of positively charged amino acids it contains.
Purity: The purified protein is >95% homogeneous (electrophoresis).
Activity: This preparation has low cytokine activity, but is fully active as a DNA binding protein or as an antigen.
Buffer & Reconstitution: the lyophilized protein once reconstituted with distilled water will be dissolved in a solution containing 50 mM HEPES pH 7.9, 500 mM NaCl, 0.5 mM DTT.
Storage: We advise to keep the product frozen at -20°C.
This product is intended for research only, and cannot be used on humans.
Publications:
- Association of High Mobility Group Box-Protein 1 and Platelet Microparticles in Patients After Hematopoietic Stem Cell Transplantation
- HMGB1 Release Induced by EV71 Infection Exacerbates Blood-Brain Barrier Disruption via VE-cadherin Phosphorylation
- [Altered expression of 15-hydroxyprostaglandin dehydrogenase in chronic rhinosinusitis with nasal polyps]
- HMGB1 induces hepcidin upregulation in astrocytes and causes an acute iron surge and subsequent ferroptosis in the postischemic brain
- HMGB1-activated tumor-associated macrophages promote migration and invasion via NF-κB/IL-6 signaling in oral squamous cell carcinoma
- The Expression of High Mobility Group Box-1 (HMG1) in the Peripheral Blood and Its Relation with Systemic Vasculitis Patients
- The translational potential of miR-26 in atherosclerosis and development of agents for its target genes ACC1/2, COL1A1, CPT1A, FBP1, DGAT2, and SMAD7
- The Anti-inflammatory Effects of HMGB1 Blockades in a Mouse Model of Cutaneous Vasculitis
- HMGB1 cleavage by complement C1s and its potent anti-inflammatory product
- HMGB1 Promotes the Release of Sonic Hedgehog From Astrocytes
- The Role of High Mobility Group Box 1 in Ischemic Stroke
- High mobility group A proteins as tumor markers
- High Mobility Group Proteins in Sepsis
- The Roles of High Mobility Group Box 1 in Cerebral Ischemic Injury
- MiR-2113 overexpression attenuates sepsis-induced acute pulmonary dysfunction, inflammation and fibrosis by inhibition of HMGB1
- Transposon dynamics in the emerging oilseed crop Thlaspi arvense
- Comparative analyses of the Smith-Magenis syndrome protein RAI1 in mice and common marmoset monkeys
- Intestinal epithelia and myeloid immune cells shape colitis severity and colorectal carcinogenesis via High-mobility group box protein 1
- Toxoplasma gondii Infection Induces High Mobility Group Box 1 Released from Mouse Macrophages
- Impact of folic acid supplementation on ischemia‒reperfusion-induced kidney injury in rats: folic acid prophylactic role revisited
- Co-existence of anti-glutamic acid decarboxylase-65 and anti-sry-like high-mobility group box receptor antibody-associated autoimmune encephalitis: A rare case report
- High Mobility Group Box 1 Gene Polymorphism and Serum High Mobility Group Box 1, Interleukin 1 Beta, and Alpha-Klotho Crosstalk in Severe COVID-19 Patients
- HO-1 attenuates testicular ischaemia/reperfusion injury by activating the phosphorylated C-jun-miR-221/222-TOX pathway
- MicroRNA-146a gene transfer ameliorates senescence and senescence-associated secretory phenotypes in tendinopathic tenocytes
- Therapeutic effects of anti-diabetic drugs on traumatic brain injury
- ASSOCIATION BETWEEN HIGH MOBILITY GROUP BOX 1 PROTEIN GENE (rs41369348) POLYMORPHISM AND IMMUNOGLOBULIN A VASCULITIS IN CHILDREN
- Mollica L et al. Glycyrrhizin binds to High Mobility Group Box 1 protein (HMGB1) and inhibits its cytokine activities. Chem Biol 2007, 14: 431-41
Download HMGB1 for Biochemestry Datasheet
Complete Name: High Mobility Group 1
Other Names: Amphoterin-1, High mobility group protein 1,HMG-1, SBP-1, high mobility group protein B1, high-mobility group box 1, Amphoterin, HMG1, HMGB1, SBP-1, recombinant HMGB1
