p-mHMG1tag plasmid (20 µg) [HM-301]
Plasmid p-HMG1tag is derived from plasmid p-mHMG1, and expresses mouse HMGB1 containing the HA epitope, for detection with anti-HA monoclonal antibody (Falciola et al. High mobility group 1 protein is not stably associated with the chromosomes of somatic cells. J Cell Biol 1997, 137: 19-26).
Mouse HMGB1 contains only 2 conservative substitutions with respect to human HMGB1.
When transfected in mammalian cells, this plasmid increases transiently the HMGB1 content 4- to 7-fold.
Other publications where this plasmid was used:
Calogero et al. The lack of chromosomal protein HMG1 does not disrupt cell growth, but causes lethal hypoglycaemia in newborn mice. Nature Genet 1999, 22: 276-80.
Aidinis et al. The RAG1 homeodomain recruits HMG1 and HMG2 to facilitate Recombination Signal Sequence binding and to enhance the intrinsic DNA-bending activity of RAG1-RAG2. Mol Cell Biol 1999, 19: 6532-42.